BiologybioRxiv
Ranked by formulaVerdict: NotableActive Learning framework for Antibody Binding Affinity Prediction
Antibody engineering campaigns must prioritise variants from large combinatorial libraries under limited assay budgets.
Key numbers
- 10 % of each full pool
- 2 % point estimate among tested
- 57.8 % mean Recall
Caveats
- Preprint; not yet peer reviewed.
VerdictWorth a reader's time today.
Abstract
Antibody engineering campaigns must prioritise variants from large combinatorial libraries under limited assay budgets. We present a Gaussian process (GP) active-learning benchmark for antibody-antigen binding affinity prediction across eight public AbBiBench assays spanning influenza hemagglutinin antibodies CR6261 and CR9114 and AAYL-series scFv libraries against the SARS-CoV-2 Spike HR2 peptide. The framework embeds variable heavy and light (VH/VL) sequences with ESM-2 650M, trains GP surrogates over compressed protein-language-model representations, and evaluates kernel, acquisition, representation, classical featurisation, and labelling-budget choices under a unified budget of 10% of each full pool with a label-blind seed and a fixed held-out evaluation split. Across the benchmark, Tanimoto+UCB has the highest mean Recall@2% point estimate among tested kernel-acquisition configurations, achieving 57.8% mean Recall@2% across five random seeds (68.3% when scored against the acquisition-eligible pool), compared with 9.6% for random selection -- a gap that is significant by a paired Wilcoxon test across datasets (p=0.0078); its point estimate is not significantly different from RQ+UCB or Tanimoto+EI at five seeds. We adopt the continuous Tanimoto kernel of \citet{gessner2024}. The same configuration recovers the largest fractions of top binders in the influenza libraries (74.2% on 3gbn_h1, 82.7% on 3gbn_h9, and 84.9% on 4fqi_h3) while exposing harder AAYL combinatorial landscapes where recall remains lower and more variable. An additional three-seed representation ablation, run under the original protocol and not repeated after the evaluation corrections, ranked ESM-2 650M highest (mean Recall@2% 0.673 ± 0.28) among ProtBert, AntiBERTy, AbLang2, ProGen2, and classical encodings. On BindingGYM, the same configuration matches or exceeds ALLM-Ab on two of three datasets using the 100-sample held-out test metric. Structure-mapped Integrated Gradients and KernelSHAP analyses recover CDR-region attribution patterns consistent with contact-derived paratope geometry, though threshold-free (AUROC) analysis shows this qualitative agreement is not matched by strong residue-level discrimination. These results support Tanimoto-kernel GP active learning as a simple, reproducible strategy for label-efficient antibody affinity optimisation with qualitative structural validation.
The editor's rubric
- Leverage3 / 5
- A method or resource many groups across the field will adopt within a year.
- Magnitude3 / 5
- Large gain: roughly 2x, or a clear new state of the art on a hard, unsaturated problem.
- Evidence4 / 5
- Strong: large scale, preregistered, independently replicated, or a well-powered randomized trial.
- Novelty2 / 5
- A new combination of known ideas.
- Trajectory3 / 5
- A clear path to scale.
- Stakes2 / 5
- Benefits a professional community (practitioners, clinicians, engineers).
Editor’s rationale
Heuristic triage from title and abstract text only, not a reading of the paper. Cues found: method (we propose); breadth (many tasks); gains (outperforms); verification (p-value, multiple benchmarks, ablations); scale (efficient); stakes (major disease).
How the score was computed
Desks place stronger verdicts first, then use this score to order work within the same verdict.
- Merit
- 5.8 / 10
- Adjusted merit
- 4.9 / 10
- Attention
- 0%
- Freshness
- 88%